mouse monoclonal anti-ionized calcium-binding adaptor molecule 1 iba1  (GeneTex)

Journal: Brain Sciences

Article Title: Liver Growth Factor Induces Glia-Associated Neuroprotection in an In Vitro Model of Parkinson´s Disease

doi: 10.3390/brainsci10050315

Figure Lengend Snippet: Liver growth factor (LGF) modulated Iba1 protein expression in cultured mesencephalic glia. ( A ) shows how 100 pg/mL of LGF treatment up-regulated Iba1 protein expression after 6 h (dots bar) and 24 h of treatment (vertical black lines bar), as compared to cultures maintained in complete medium (CM) (black bar) and defined medium (DM) (white bar) for 6 h. Iba1 protein levels were also increased in cultures maintained in DM for 24 h ( A ) (horizontal black lines bar). GFAP expression was also up-regulated after 24 h of DM ( B ) (horizontal black lines bar) and DM-LGF ( B ) (vertical black lines bar). ( C ) shows representative bands of Iba1 and GFAP. Results represent the mean ± SEM of 4 ( A ) and 5 ( B ) independent experiments. ** p ≤ 0.01 and *** p ≤ 0.001 vs. CM, + p ≤ 0.05, +++ p ≤ 0.001 vs. 6 h DM (ANOVA and Newman–Keuls).

Article Snippet: Membranes were soaked in a blocking solution (0.1 M PBS and 5% dry skimmed milk, pH 7.4) and incubated with the following primary antibodies diluted in 0.1 M PBS and 1% dry skimmed milk at pH 7.4: For the immunodetection of markers of microglia, we used a mouse monoclonal anti-ionized calcium binding adaptor molecule 1 (Iba1) (17 kDa) (1:500, Millipore), and for astrocytes, we used anti-GFAP (50 kDa) (1:5000).

Techniques: Expressing, Cell Culture